nrdE [2019-06-19 14:16:40]

ribonucleoside-diphosphate reductase (alpha subunit), control of DNA/cell mass ratio

Locus: BSU17380

Isoelectric point: 5.93

Molecular weight: 80.50 kDa

Protein length: 700 aa

Gene length: 2103 bp

Function: synthesis of deoxyribonucleoside triphosphates

Product: ribonucleoside-diphosphate reductase (alpha subunit)

Essential: yes

E.C.: 1.17.4.1

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of nucleotides/ other
• Groups of genes → Essential genes

Gene

Coordinates: 1,868,969 1,871,071

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
• required for stationary-phase mutagenesis PubMed

Protein family

• ribonucleoside diphosphate reductase large chain family (according to Swiss-Prot)

Paralogous protein(s)

• NrdEB

Kinetic information

• Steady-state kinetics PubMed

Effectors of protein activity

• ATP stimulates CDP and UDP reduction PubMed
• dATP at concentrations up to ~10 M stimulate CDP and UDP reduction, while higher concentrations inhibit this activity, inhibition by dATP is enhanced by binding of dAMP to the N-terminal domain of NrdE PubMed
• TTP stimulates GDP reduction PubMed
• dGTP stimulates ADP reduction PubMed

Structure

• 6CGL (pH 4, dAMP bound) PubMed
• 6CGM (pH 7, apo-protein) PubMed
• 6CGN (pH 7, dAMP bound) PubMed
• 6MT9 (TTP in substrate specificity site, ADP in inhibitory site, and ATP in activity site) PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: nrdI-nrdE-nrdF-ymaB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • NrdR: repression, (predicted) PubMed, in NrdR regulon
  • PerR: transcription repression, PubMed, in PerR regulon

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

  Additional information

  • A ncRNA is predicted between 'ymzA' and 'nrdI' PubMed

• Genes: ymzA-nrdI-nrdE-nrdF-ymaB
  Description: PubMed

Labs working on this gene/protein

• Elisabeth Härtig, Braunschweig, Germany Homepage

References

Reviews

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Original Publications

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